Nexera series - Configuration

Ultra High Performance Liquid Chromatograph

System configuration

System configuration

Detector
Pump
Autosampler
Mobile Phase Monitor
System Controller
Degassing Unit
Column Oven
Plate Changer

System Controller

SCL-40

 
 
 

CBM-40

  SCL-40 CBM-40 CBM-40lite
Monitor Touch panel
LabSolutions™
Web monitor		 LabSolutions
Web monitor		 LabSolutions
Web monitor
Connectable unit
 		 Solvent delivery unit: max. 4, Autosampler: 1, Column oven: max. 4, Detector: max 2, etc.
Number of connectable units 8 (Using option: 12) 4 (Excluding built-in solvent delivery unit)
Event input/output Input: 1, output: 2
Analog board
 		 Up to two channels (option) Up to one channel (option) ̶
Communication Ethernet
Reservoir tray Built-in ̶
Dimensions, weight W 260×D 500×H 140 mm、6 kg W 260×D 500×H 72 mm、5 kg ̶
Operating temperature range 4 to 35°C
Power supply AC 100–240 V, 50 VA, 50/60 Hz Supplied from solvent delivery unit

Solvent Delivery Pump

 

LC-40B XR

 

LC-40B X3

  LC-40D
 		 LC-40D XR
LC-40B XR		 LC-40D XS LC-40D X3
LC-40B X3
Pumping method Parallel-type double plunger (approx. 10 μL/1 stroke)
Allowable maximum pressure 44 MPa 70 MPa 105 MPa 130 MPa
Flow rate settings range 0.0001-5.0000 mL/min
（1.0-44 MPa）
5.0001-10.0000 mL/min
（1.0-22 MPa）		 0.0001-3.0000 mL/min
（1.0-70 MPa）
3.0001-5.0000 mL/min
（1.0-44 MPa）
5.0001-10.0000 mL/min
（1.0-22 MPa）		 0.0001-3.0000 mL/min
（1.0-105 MPa）
3.0001-5.0000 mL/min
（1.0-80 MPa）
5.0001-10.0000 mL/min
（1.0-22 MPa）		 0.0001-3.0000 mL/min
（1.0-130 MPa）
3.0001-5.0000 mL/min
（1.0-80 MPa）
5.0001-10.0000 mL/min
（1.0-22 MPa）
Flow rate accuracy ≤± 1% or ± 2 μL/min, whichever greater
(under specified conditions)		 ≤± 1% (under specified conditions)
Flow rate accuracy ≤ 0.06% RSD or 0.02 minSD, whichever greater
Gradient mode
 		 High-pressure gradient (2 or 3 solvents) Quaternary low-pressure gradient High-pressure gradient (2 solvents (LC-40B XR standard) or 3 solvents) Quaternary low-pressure gradient (Only available for LC-40D XR) High-pressure gradient (2 or 3 solvents) Quaternary low-pressure gradient High-pressure gradient (2 solvents (LC-40B X3 standard) or 3 solvents) Quaternary low-pressure gradient (Only available for LC-40D X3)
Gradient range of set concentrations 0 to 100% (0.1% step)
Gradient range of set concentrations ± 0.5% (under specified conditions)
Wetted materials SUS316L, Hastelloy® C, PEEK, PTFE, Sapphire, Ruby SUS316L, Hastelloy C, PEEK, PE, Sapphire, Ruby
Available pH range 1 to 14
Automatic rinsing kit Option Standard equipment
Degassing unit 1 unit connectable LC-40D XR:1 unit connectable LC-40B XR: 2 units connectable 1 unit connectable
LC-40D X3:
1 unit connectable
LC-40B X3:
pre-installed (5 port builtin), 1 unit connectable
Dimensions  W 260×D 500×H 140 mm LC-40D X3：
W 260×D 500×H 140 mm
LC-40B X3：
W 260×D 500×H 210 mm
Weight 10 kg LC-40D XR：10 kg
LC-40B XR：13 kg		 12 kg LC-40D X3：12 kg
LC-40B X3： 21 kg
Operating temperature range 4 to 35°C
Power supply AC 100-240 V、50/60 Hz
150 VA LC-40D XR：150VA
LC-40B XR：180 VA		 150 VA LC-40D X3：150 VA
LC-40B X3：180 VA

Degassing Unit

DGU-403

 

  DGU-403 DGU-405
Number of degassed solvents 3 5
Degassed flow line capacity 400 μL/1 line
Dimensions , weight W 260×D 500×H 72 mm、4 kg
Operating temperature range 4 to 35°C
Power supply Supplied from solvent delivery unit

Autosampler

SIL-40C XR

 

  SIL-40
SIL-40C		 SIL-40 XR
SIL-40C XR		 SIL-40C XS SIL-40C X3
Injection method
 		 Total-volume Injection (standard), loop injection (optional)
Allowable
maximum pressure
 44 MPa 80 MPa 105 MPa 130 MPa
Injection volume 0.1-100 μL 0.1-50 μL
0.1 to 2000 μL (optional)
Injection volume
accuracy
 ≤ ± 1% (5 μL injection, n = 20)
Linearity ≥ 0.9999
Injection cycle time ≤ 6.7 seconds (under specified conditions)
Samples for processing   288（ microtiter plate, 96 well × 3 plates）
  1152（ microtiter plate, 384 well × 3 plates）
252（ 1 mL sample vial, 84 × 3 plates）
  162（ 1.5 mL sample vial, 54 × 3 plates）
84（ 4 mL sample vial, 28 × 3 plates）
  36（ 10 mL sample vial, 12 × 3 plates）
     72（ 1.5 mL micro tube, 24 × 3 plates)） 
Injection volume reproducibility
 		 RSD≦1.0％ （0.5-0.9 μL）
RSD≦0.5％ （1.0-1.9 μL）
 RSD≦0.25％（ 2.0-4.9 μL）
           RSD≦0.15％（ More than 5.0 μL）
       RSD＜0.5％ （typically, 0.5 μL）
         RSD＜0.25％（ typically, 1.0 μL）
Carryover
 		 ≤ 0.0025% (without rinse)
≤0.0005％
(with rinse, typically）
（under specified conditions）		 ≤ 0.0015％（without rinse）
≤ 0.0003％（with rinse, typically)）
（under specified conditions)
Dip rinsing outside the needle
and injection port rinsing
 		 Standard equipment
Pumping rinse
outside the needle
 Option Standard equipment
Internal rinsing (3 dil) Option Standard equipment
Sample cooler
 		 SIL-40：None
SIL-40C：Standard equipment
(Air-circulation temperature
control type)
 		 SIL-40 XR：None
SIL-40C XR：Standard equipment
(Air-circulation temperature
control type)
 		 Standard equipment
(Air-circulation temperature control type)
Sample cooler
temperature setting range
 
4 to 45°C
(Room temperature needs to be less than 30°C and humidity needs to be less than 70% to set 4°C)
Sample cooler
temperature accuracy
 		 ±2 ℃（sensor position ± 0.5°C）
Wetted material SUS316L, DLC, PEEK, GFP, PTFE, FEP, ETFE, sapphire, ceramics, PPS, FFKM
Available pH range 1 to 14
Dimensions  W 260×D 500×H 280 mm（SIL-40C/40C XR/40C XS/40C X3Protrusion adds 140 mm to the depth）
Weight SIL-40：17 kg
SIL-40C：24 kg
 		 SIL-40 XR：17 kg
SIL-40C XR：24 kg		 24 kg
Operating
temperature range
 4 to 35 ℃
Power supply Cooler model AC 100-240 V, 50/60 Hz
Non cooler model SIL-40：150 VA
SIL-40C：400 VA		 SIL-40 XR：150 VA
SIL-40C XR：400 VA
 
ー

Column Oven

CTO-40C

CTO-40S

  CTO-40C CTO-40S
Temperature control type Forced air circulation
Cooling Method Electronic cooling
Temperature control range Room temperature − 10°C to 100°C Room temperature − 10°C to 85°C
Temperature accuracy ±0.5 ℃ ±0.8 ℃
Temperature precision 0.1 ℃（±0.05 ℃） 0.2 ℃（±0.1 ℃）
Containable column size and
number		 Up to 250 mm L. column × 6
or 300 mm L. column × 3		 Up to 100 mm L. column × 6
or 300 mm L. column × 3
Dimensions, weight W 260×D 500×H 415 mm、21 kg W 130×D 500×H 553 mm、15 kg
Operating temperature range 4 to 35°C
Power supply AC 100～120 V/AC 220-240 V（Automatic switching）
400 VA、50/60 Hz		 AC 100-240 V、300 VA、50/60 Hz

Detector

SPD-40V

  SPD-40 SPD-40V
Light source  Deuterium (D2) lamp Deuterium (D2) lamp, tungsten lamp
Wavelength range 190 to 700 nm 190 to 1000 nm
Bandwidth 8 nm
Wavelength accuracy ≤ ± 1 nm±1 nm
Wavelength reproducibility ≤ ± 0.1 nm
Drift ≤ 0.1 × 10−3 of AU/h (under specified conditions)
Noise 1 Wavelength mode: ≤ 4.0 × 10−6 AU, 2 Wavelength mode: ≤ 10.0 × 10−6 AU (under specified conditions)
Linearity 2.5 AU (under specified conditions)
Standard flow cell Optical path length: 10 mm, Cell volume: 12 μL, Pressure: 12 MPa
Material of wetted parts: SUS316L, PFA, quartz, PEEK
Cell temperature control range 19 to 50°C, 1°C Step
Optional flow cell
 		 UHPLC cell (optical path length: 10 mm, cell volume: 8 μL, equipped with temperature control function)
Semi-micro cell (optical path length: 5 mm, cell volume: 2.5 μL, equipped with temperature control function)
Conventional cell (optical path length: 10 mm, cell volume: 12 μL, equipped with temperature control function)
Inert cell (optical path length: 10 mm, cell volume: 12 μL, equipped with temperature control function)
Preparative cell (optical path length: 0.1/0.2/0.5 mm, cell volume: 0.8/1.6/4.0 μL)
Micro flow cell (optical path length: 3 mm, cell volume: 0.21 μL)
Maximum pressure cell (optical path length: 10 mm, cell volume: 12 μL)
Available pH range 1 to 13 (Cell quartz might be damaged by a mobile phase of pH >10.)
Dimensions , weight W 260 × D 500 × H 140 mm, 11 kg
Operating temperature range 4 to 35°C
Power supply AC 100–240 V, 150 VA, 50/60 Hz

Photodiode Array Detector

SPD-M40

  SPD-M40
Light source Deuterium (D2) lamp, Tungsten lamp
Number of diode elements 1024
Wavelength range 190 to 800 nm
Wavelength accuracy ≤ ±1 nm
Wavelength reproducibility ≤ ±0.1 nm
Slit width 1.2 nm、8 nm
Spectral resolution ≤ ± 1.4 nm
Drift ≤ 0.4×10－3 AU/h (under specified conditions)
Noise ≤ 4.5 × 10−6 AU (under specified conditions)
Linearity 2.5 AU (under specified conditions)
Standard flow cell Optical path length: 10 mm, Cell volume: 12 μL, Pressure: 12 MPa
Material of wetted parts: SUS316L, PFA, quartz, PEEK
Cell temperature control range 19 to 50°C, 1°C Step
Optional flow cell
 		 UHPLC cell (optical path length: 10 mm, cell volume: 8 μL, equipped with temperature control function)
Semi-micro cell (optical path length: 5 mm, cell volume: 2.5 μL, equipped with temperature control function)
Conventional cell (optical path length: 10 mm, cell volume: 12 μL, equipped with temperature control function)
Inert cell (optical path length: 10 mm, cell volume: 12 μL, equipped with temperature control function)
Preparative cell (optical path length: 0.1/0.2/0.5 mm, cell volume: 0.8/1.6/4.0 μL, equipped)
Micro flow cell (optical path length: 3 mm, cell volume: 0.21 μL)
Maximum pressure cell (optical path length: 10 mm, cell volume: 12 μL)
Available pH range 1 to 13 (Cell quartz might be damaged by a mobile phase pH >10.)
Dimensions , weight W 260 × D 500 × H 140 mm, 10 kg
Operating temperature range 4 to 35°C
Power supply AC 100–240 V, 180 VA, 50/60 Hz

Capillary cell type Photodiode Array Detector

  SPD-M30A
Light source Deuterium (D2) lamp
Number of diode elements 1024
Wavelength range 190 to 700 nm
Wavelength accuracy ≤ ±1 nm
Wavelength reproducibility ≤ ±0.1 nm
Slit width 1 nm, 8 nm
Spectral resolution ≤ 1.4 nm
Drift ≤ 4.0 × 10−6 AU (under specified conditions)
Noise ≤ 4.0 × 10−6 AU (under specified conditions)
Linearity 2.0 AU (under specified conditions)
Cell Standard cell: Optical path length: 10 mm, Capacity: 1 μL, Pressure: 8 MPa
Optional high-sensitivity cell: Optical path length: 85 mm, Capacity: 9 μL, Pressure: 8 MPa
Dimensions, weight W 260×D 500×H 140 mm,12 kg
Operating temperature range 4 to 35°C
Power supply AC 100–240 V, 150 VA, 50/60 Hz

Spectrofluorometric Detector

  RF-20A RF-20AXS
Light source
 		 Xenon lamp Xenon lamp
Low-pressure mercury lamp
(to check wavelength accuracy)
Wavelength range 200 to 650 nm 200 to 750 nm
Spectral bandwidth 20 nm
Wavelength accuracy
 		 ±2 nm
 
Wavelength precision
 ±0.2 nm
S/N
 		 Water Raman peak S/N ≥ 200
Low background S/N ≥ 9000		 Water Raman peak S/N ≥ 2000
Low background S/N ≥ 12000
Range of cell temperature control ̶ Room temperature − 10°C to 40°C, 1°C step
S/N
 Water Raman peak S/N ≥1200
Low background S/N ≥ 9000		 Water Raman peak S/N ≥ 2000
Low background S/N ≥ 12000
Cell Standard conventional cell: volume 12 μL, maximum pressure 2 MPa
Optional semi-micro cell: volume 3 μL, maximum pressure 2 MPa
Function Simultaneous measurement of four wavelengths, Wavelength scanning
Dimensions  W 260×D 500×H 210 mm
Weight 16 kg 18 kg
Operating temperature range 4 to 35°C
Power supply AC 100–240 V, 400 VA, 50/60 Hz

Differential Refractive Index Detector

  RID-20A
Measurement range 1 to 1.75 RIU
Noise ≤ 2.5×10－9 RIU
Drift ≤ 1×10－7 RIU/h
Range A mode: 0.01 × 10−6 to 500 × 10−6 RIU
P, L-mode: 1 × 10−6 to 5000 × 10−6 RIU
Response
 0.05 to 10 sec, 10 steps
Polarity – Change Available
Zero adjustment Auto zero, Optical zero, Fine zero
Maximum flow rate
20 mL/min (150 mL/min in option)
Range of cell temperature control 30 to 60°C
Cell Volume 9 μL, Maximum pressure 2 MPa
Dimensions, weight W 260 × D 420 × H 140 mm, 12 kg
Operating temperature range 4 to 35°C
Power supply AC 100–240 V, 150 VA, 50/60 Hz

Conductivity Detector

  CDD-10Avp
Cell volume 0.25 μL
Cell constant 25 μS·cm⁻¹
Material of wetted parts PEEK, SUS316
Maximum use pressure 2.9 MPa（30 kgf/cm2）
Response
 0.05 to 10 s, 10 steps
Zero adjustment Auto-zero function, Baseline-shifting function
Dimensions, weight W 260 × D 420 × H 140 mm, 6 kg
Operating temperature range
 4 to 35°C
Power supply AC 100–240 V, 250 VA, 50/60 Hz

Evaporative Light-Scattering Detector

  ELSD-LT II ELSD-LT III
Nebulizing method Siphon Splitting Siphon Splitting
Light source LED Semiconductor laser
Detector Photomultiplier Photodiode
Temperature Setting Range Room temperature + 5°C to 80°C Room temperature to 100 °C
Nebulizer Gas
 Nitrogen or air* Nitrogen or air*
Gas flow rate, gas pressure Up to 3.0 mL/min, up to 450 kPa Up to 3.0 mL/min, up to 450 kPa
Mobile Phase Flow Rate
(Standard Nebulizer)		 0.2 to 2.5 mL/min 0.2 to 2 mL/min
Analog output
 0 to 1 V 0 to 1 V
Dimensions W 260×D 550×H 450 mm W 250×D 530×H 330 mm
weight 20 kg 15.5 kg
Operating temperature range 5 to 40°C 4 to 35°C
Operation humidity range ≤ 80% (Room temperature + 5°C to 31°C),
 ≤ 50% (Room temperature + 31°C to 40 °C)		 20 to 85 %
Power supply AC 100 V, 210 VA, 50/60 Hz 100 to 240 VAC, 1.2 A (max), 50/60 Hz

* Supply gas at a pressure of about 350 kPa.
   An air compressor may also be used.
   A filter (P/N: 228-45528-92) is also available for filtering out moisture and other matter from the compressor.
   For more details, contact a Shimadzu sales representative. 

Mobile Phase Monitor  MPM-40 ANALYTICAL INTELLIGENCE

Mobile Phase Monitor
  • Monitor mobile phase levels remotely from a PC or smartphone
  • Avoid running out of mobile phase or rinse solution mid-analysis
  • Fits a range of bottle sizes from 1 to 5L

 

    Description
Mobile Phase Monitor (Controller)   This is a controller to connect the bottle holders.
It can connect two reserve trays of bottle holders.
Mobile Phase Monitor (1L bottle holder)

1L bottle holder

 One holder can accommodate two 1L bottles.
Mobile Phase Monitor (Large bottle holder)

Large bottle holder

 One holder can accommodate one large bottle.

※ The large bottle holder can hold bottles up to a diameter of approx. 180 mm.
※ To change between the two holder types, a field engineer will need to carry out the modifications.

 

Versatile layout can accommodate different bottle combinations

Two trays of bottles can be monitored from one MPM controller.

Large bottle holder ×1 , 1L bottle holder ×1

  • Large bottle holder ×1
  • 1L bottle holder ×1

1L bottle holders ×3

  • 1L bottle holders ×3

Large bottle holders ×2

  • Large bottle holders ×2
 
 
 
ANALYTICAL INTELLIGENCE

ANALYTICAL INTELLIGENCE

Automated support functions utilizing digital technology, such as M2M, IoT, and Artificial Intelligence (AI), that enable higher productivity and maximum reliability.

Plate Changer

  PLATE CHANGER
Samples for processing
(includes two plates of autosampler)
1 PLATE CHANGER
1536 (microtiter plate, 96 well × 16 plates)
864 (deep-well plate, 96 well × 9 plates)
6144 (microtiter plate, 384 well × 16 plates) 
3456 (deep-well plate, 384 well × 9 plates)
756 (1 mL sample vial, 84 × 9 plates)
486 (1.5 mL sample vial, 54 × 9 plates)
252 (4 mL sample vial, 28 × 9 plates)
108 (10 mL sample vial, 12 × 9 plates)
3 PLATE CHANGERS
4224 (microtiter plate, 96 well × 44 plates)
2208 (deep-well plate, 96 well × 23 plates)
16896 (microtiter plate, 384 well × 44 plates)
8832 (deep-well plate, 384 well × 23 plates)
1932 (1 mL sample vial, 84 × 23 plates)
1242 (1.5 mL sample vial, 54 × 23 plates)
644 (4 mL sample vial, 28 × 23 plates)
276 (10 mL sample vial, 12 × 23 plates)
Sample cooler
temperature setting range		 Air-circulation temperature control type, 4 to 45°C
(Room temperature needs to be less than 30°C and humidity needs to be less than 70% to set 4°C)
Dimensions, weight W 170 × D 500 × H 560 mm (Protrusion adds 140 mm to the depth), 26 kg
Operating temperature range 4 to 35°C
Power supply AC 100−240 V, 400 VA, 50/60 Hz
 
 

High-Pressure Gradient Using Dual Pump, Photodiode Array Detector

P/N Description Q'ty
228-65500-46/58 SCL-40 1
228-65078-46/58 LC-40B X3 1
228-65120-46/58 SIL-40C X3 1
228-65201-46/58 CTO-40S 1
228-65302-46/58 SPD-M40 1
228-64724-41 UHPLC Cell 1
228-72652-42 Mixer MR 40 μL
(with a mixer recognition device)		 1
228-70254-42 Piping Kit B, for High-Pressure GE ID0.1 1
228-70247-43 Nexera Series Cable Installation Kit, C 1
Power Cord, Length: 2.5 m 5
228-57647-43 Hand Tool Kit 1

High-Pressure Gradient Using Dual Pump, Plate Changer, Photodiode Array Detector

P/N Description Q'ty
228-65500-46/58 SCL-40 1
228-65078-46/58 LC-40B X3 1
228-65120-46/58 SIL-40C X3 1
228-65110-46/58 Plate Changer 1
228-65201-46/58 CTO-40S 1
228-65302-46/58 SPD-M40 1
228-64724-41 UHPLC Cell 1
228-72652-42 Mixer MR 40 μL
(with a mixer recognition device)		 1
228-70254-42 Piping Kit B, for High-Pressure GE ID0.1 1
228-70247-43 Nexera Series Cable Installation Kit, C 1
Power Cord, Length: 2.5 m 6
228-57647-43 Hand Tool Kit 1

High-Pressure Gradient Using Two Pumps, Photodiode Array Detector

P/N Description Q'ty
228-65500-46/58 SCL-40 1
228-65076-46/58 LC-40D X3 2
228-65019-58 DGU-405 1
228-65120-46/58 SIL-40C X3 1
228-65202-46/58 CTO-40C 1
228-65302-46/58 SPD-M40 1
228-64724-41 UHPLC Cell 1
228-72652-42 Mixer MR 40 μL
(with a mixer recognition device)		 1
228-70254-42 Piping Kit B, for High-Pressure GE ID0.1 1
228-70247-43 Nexera Series Cable Installation Kit, C 1
Power Cord, Length: 2.5 m 6
228-57647-43 Hand Tool Kit 1

High-Pressure Gradient Using Dual Pump, UV-VIS Detector

P/N Description Q'ty
228-65500-46/58 SCL-40 1
228-65078-46/58 LC-40B X3 1
228-65120-46/58 SIL-40C X3 1
228-65202-46/58 CTO-40C 1
228-65313-46/58 SPD-40V 1
228-64724-42 UHPLC Cell 1
228-72652-42 Mixer MR 40 μL
(with a mixer recognition device)		 1
228-70254-42 Piping Kit B, for High-Pressure GE ID0.1 1
228-70247-44 Nexera Series Cable Installation Kit, D 1
Power Cord, Length: 2.5 m 5
228-57647-43 Hand Tool Kit 1

Low-Pressure Gradient, Photodiode Array Detector

P/N Description Q'ty
228-65500-46/58 SCL-40 1
228-65076-46/58 LC-40D X3 1
228-65016-46/58 Low-Pressure Gradient Kit 1
228-65019-58 DGU-405 1
228-65120-46/58 SIL-40C X3 1
228-65201-46/58 CTO-40S 1
228-65302-46/58 SPD-M40 1
228-64724-41 UHPLC Cell 1
228-65020-41 Mixer Installation Kit, MR 40 μL LPGE (with a mixer recognition device) 1
228-70247-42 Nexera Series Cable Installation Kit, B 1
Power Cord, Length: 2.5 m 5
228-57647-43 Hand Tool Kit 1
228-36573 1/16"NYLON COUPLING 2
228-56687-42 SF NUT,1/8 4
228-56687-43 SF FERRULE,1/8 4