User interviews December 2020
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LC/MS/MS Method Package for Mycotoxins
For LabSolutions™ LCMS Software
Mycotoxins are highly toxic chemical byproducts from fungi commonly found in crops, and residual content in foods are regulated the world over. This method package provides simultaneous multi-component analysis conditions for mycotoxins subject to the regulations in Japan, the USA, the EU, and China.
Analytical Methods for 27 Mycotoxins
This package contains seven different analytical method conditions and fully optimized MRM transitions for a total of 27 different mycotoxins. Rapid analytical methods are included for a 25 mycotoxin screen, 16 mycotoxins using high-sensitivity conditions, 10 mycotoxins using APCI, and specialized methods for trichothecenes, fumonisins, aflatoxins, and patulin.
Analytical Method for 25 Mycotoxins in 15 Minutes
HPLC Conditions
Column ： Mastro™ PFP2 (Shimadzu GLC)
(2.1 mmI.D. x 150 mmL, 3 µm)
Mobile Phase A ： 10 mmol/L aqueous ammonium acetate
Mobile Phase B ： Methanol (2 % acetic acid)
Flowrate ： 0.4 mL/min
Column Temp. ： 40 °C
MS Conditions
Ionization Mode ： ESI（+/-）
Nebulizer Gas Flowrate ： 2 L/min
Drying Gas Flowrate ： 10 L/min
Heating Gas Flowrate ： 10 L/min
Interface Temp. ： 300 °C
DL Temp. ： 250 °C
Heat Block Temp. ： 500 °C
High-Speed Simultaneous Analysis of 25 Mycotoxins
Compound List for Each Method
| Method name | Detail | Analysis time | |
|---|---|---|---|
| A | A_MRM_25_PFP_15m | Simultaneous analysis of 25 mycotoxins | 15 min |
| B | B_MRM_16_ODS_30m | Simultaneous analysis with high sensitivity | 30 min |
| C | C_MRM_1_PAT_ODS_10m | Patulin analysis | 10 min |
| D | D_MRM_6_AF_ODS_30m | Aflatoxins analysis | 30 min |
| E | E_MRM_4_DONNIV_ODS_12m | Trichothecenes analysis | 12 min |
| F | F_MRM_3_FB_ODS_15m | Fumonisins analysis | 15 min |
| G | G_MRM_10_ODS_33m | 10 mycotoxins using APCI | 33 min |
- AF B1: aflatoxin B1
- AF B2: aflatoxin B2
- AF G1: aflatoxin G1
- AF G2: aflatoxin G2
- AF M1: aflatoxin M1
- AF M2: aflatoxin M2
- DON: deoxynivalenol
- 3ADON: 3-acetyl-deoxynivalenol
- 15ADON: 15-acetyl-deoxynivalenol
- NIV: nivalenol
- FUX: 4-acetyl-nivalenol
- FB1: fumonisin B1
- FB2: fumonisin B2
- FB3: fumonisin B3
- OTA: ochratoxin A
- PAT: patulin
- T-2: T-2 toxin
- HT-2: HT-2 toxin
- ZEN: zearalenone
- α-ZEL: α-zearalenol
- β-ZEL: β-zearalenol
- α-ZAL: α-zearalanol
- β-ZAL: β-zearalanol
- DAS: diacetoxy-scirpenol
- NEO: neosolaniol
- D3G: deoxynivalenol-3-glucoside
- STE: sterigmatocystin
Note
- LabSolutions LCMS Ver. 5.89 or later is required.
- Matsuro is a trademark of Shimadzu GLC Ltd.
News / Events
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LCMS Food Safety Application is now available.
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Application Note: "Analysis of Pharmaceuticals' Impurity - Regulations and Analysis for Carcinogenic Substances -"
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Application Note: "Various Analysis Techniques for Organic Acids and Examples of Their Application"
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Shimadzu has released the Metabolites Method Package Suite.
Provides ready-to-use methods for over 1900 metabolites
This suite allows comprehensive analysis of over 1900 metabolites without the need for investigation of separation conditions, MRM optimization or parameter settings. -
Direct Probe Ionization Mass Spectrometer - APPLICATION NOTEBOOK -
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