User interviews December 2020
Our field of activity is contract research and testing for life sciences industries including pharma, biotech, nutraceuticals, environmental sciences, food&agri testing, and diagnostics.
For LabSolutions™ LCMS Software
Most important amino acids exist as stereoisomers. D- and L- forms of mirror image isomers, or enantiomers, are named according to their activity on polarized light. By using CROWNPAK CR-I(+) and CR-I(-) columns with chiral stationary phases, the D- and L-forms of amino acids can be analyzed separately. With CR-I(+) elution order is from D- to L-, and with CR-I(-) the elution order is reversed.
With conventional chiral amino acid analysis, it is necessary to perform derivatization or use very long run times. With this method package, derivatization is not necessary, and high-sensitivity analysis can be performed in a short period of time, bringing efficiency to the chiral separations laboratory.
The physicochemical properties of Glutamine and Lysine, Isoleucine and allo-Isoleucine, Threonine and allo-Threonine are extremely similar. They have virtually the same MS/MS fragmentation patterns, and share many of their MRM transitions. Chromatographic separation is therefore required to analyze them individually by LC-MS/MS. Even if there is coelution on the CR-I(+) column, confirmation can be made by automated switching to a secondary CR-I(-) column.
Analysis of D/L-Isoleucine, D/L-allo-Isoleucine, and D/L-Leucine |
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Analysis of D/L-Threonine, and D/L-allo-Threonine |
Precautions
1. DL-Proline is a secondary amine, so it cannot be separated with these analysis conditions.
2. LabSolutions LCMS Ver. 5.86 or later is required.
The analysis method of this method package was developed based on the reserach of the Fukusaki Lab, Division of Science and Biotechnology, Graduate School of Engineering, Osaka University.
Reference: Nakano,Y., Konya,Y., Taniguchi,M., Fukusaki,E., Journal of Bioscience and Bioengineering , 123, 134-138 (2016)
LabSolutions and LCMS are trademarks of Shimadzu Corporation.
CROWNPAK is a registered trademark of Daicel Corporation.
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Applications | Creation Date |
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2019-06-03 |
User interviews December 2020
Our field of activity is contract research and testing for life sciences industries including pharma, biotech, nutraceuticals, environmental sciences, food&agri testing, and diagnostics.
LCMS Food Safety Application is now available.
Application Note: "Analysis of Pharmaceuticals' Impurity - Regulations and Analysis for Carcinogenic Substances -"
Application Note: "Various Analysis Techniques for Organic Acids and Examples of Their Application"
Shimadzu has released the Metabolites Method Package Suite.
Provides ready-to-use methods for over 1900 metabolites
This suite allows comprehensive analysis of over 1900 metabolites without the need for investigation of separation conditions, MRM optimization or parameter settings.
Direct Probe Ionization Mass Spectrometer - APPLICATION NOTEBOOK -