LC/MS/MS Method Package for Bile Acids

Bile acids are primarily produced through the breakdown of cholesterol in the liver. Primary bile acids can then be conjugated with taurine or glycine and/or converted into secondary bile acids by gut bacteria. Their role as digestive surfactants to promote the absorption of lipids is well-known, but they also act as hormones in the regulation of various metabolic pathways.

Enzymatic assays or a total bile acid test have long been used to investigate liver functions. However, more detailed profiling of bile acids can provide additional information about pathologies in clinical research.