LC/MS/MS Method Package for Lipid Mediators - Features

Lipid mediators (bioactive lipids) have important physiological functions and have been associated with allergies, thrombosis and lifestyle-related diseases. This method package provides a simultaneous analysis method that encompasses Retention Time Correcting Tool Supports Identification of Isomers The Retention Time Correcting Tool available in this version simplifies retention time correction, enabling precise identification of isomers that cannot be distinguished by MRM. The 196 compounds are divided into 18 groups based on their properties, and internal standard samples have been chosen for each group, making it possible to correct for quantitation errors that may arise, such as during solid phase extraction. UFMS Technology Covers a Wide Range of Compound Groups Fatty acids are usually detected with ESI-, but for a few important lipid mediators such as anandamide (AEA), ESI+ is preferred. Conventional joint analysis methods use only unipolar analysis. But the LCMS-8060 (NX) is capable of high-speed polarity reversal in just 5 ms, allowing many more types of compounds to be analyzed simultaneously. Compounds listed for joint analysis include 100 arachidonic acid derivatives, 26 EPA derivatives, 23 DHA derivatives, 11 ethanolamides, and 36 others, including other fatty acid metabolites and platelet-activating factor (PAF). totally 214 compounds, which include 196 compounds of lipid mediators derived from arachidonic acid cascade and 18 internal standard compounds.

The Retention Time Correcting Tool available in this version simplifies retention time correction, enabling precise identification of isomers that cannot be distinguished by MRM. The 196 compounds are divided into 18 groups based on their properties, and internal standard samples have been chosen for each group, making it possible to correct for quantitation errors that may arise, such as during solid phase extraction.

Fatty acids are usually detected with ESI-, but for a few important lipid mediators such as anandamide (AEA), ESI+ is preferred. Conventional joint analysis methods use only unipolar analysis. But the LCMS-8060 (NX) is capable of high-speed polarity reversal in just 5 ms, allowing many more types of compounds to be analyzed simultaneously. Compounds listed for joint analysis include 100 arachidonic acid derivatives, 26 EPA derivatives, 23 DHA derivatives, 11 ethanolamides, and 36 others, including other fatty acid metabolites and platelet-activating factor (PAF).

LC/MS/MS Method Package for Lipid Mediators Ver. 3

Remarks and Precautions
LabSolutions LCMS Ver. 5.99SP2 or later and LabSolutions Insight Ver. 3.7 or later are required.

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