Last update: January 28, 2021

    Plunger assembly
    P/N Appearance Qty Material Remarks Applicable model Links Applicable models
    228-52069-44 1 Sapphire With diaphragm 40DXR
    40DXS
    40DX3
    40BXR
    40BX3
    228-35009-93 1 Sapphire → External view 20AT
    20Ai
    optional for 10ATvp
    228-20284-91 1 Sapphire 228-17019-93 with holder
    → External view
    20AR
    6AD
    3 Sapphire → External view 20AR
    6AD
    228-17019-93 1 Sapphire → External view 20AR
    6AD
    228-32654-92 1 Zirconia With a holder *1)
    → External view
    optional for 10Ai
    228-32654-91 1 Sapphire With a holder *1)
    → External view
    10Ai
    228-35009-92 1 Zirconia *2)
    → External view
    10ATvp
    228-35281-91 1 Zirconia 228-35601-91 with holder and O-ring. *2)
    → External view
    10ADvp
    228-70428-41 1 Sapphire Mikros
    228-35601-91 1 Zirconia *2)
    → External view
    10ADvp
    228-35601-93 1 Sapphire → External view 30ADSF
    20AB
    20AD
    20ADXR
    20ADSP
    20ADnano
    2010
    2010HT
    optional for 10ADvp
    228-35281-95 1 Sapphire 228-35601-93 with holder
    → External view
    30ADSF
    20AB
    20AD
    20ADXR
    20ADSP
    20ADnano
    2010
    2010HT
    optional for 10ADvp
    228-51269-41 1 Sapphire → External view 20AP
    228-52069-94 1 Sapphire With diaphragm
    → External view
    30AD
    228-35281-97 1 Sapphire With diaphragm
    → External view
    2040
    2030
    228-16837-91 1 Sapphire With a holder *1)
    → External view
    8A

    *1) This plunger has a plunger fixing holder as an integrated part and is finely adjusted. A plunger assembly is not provided as a single part.
    *2) The color of the current plunger is white. Plungers provided up to April 2002 were cream-colored and ones provided up to July 1998 were white.

    Changing a material of a plunger seal may increase the service life depending on the type of the mobile phase used.

    Plunger Seal

    Changing a material of a plunger seal may increase the service life depending on the type of the mobile phase used. When changing a material of a seal, seats and other parts are also necessary.
    For details, click here.

    ■ Material Indication
    GFP, GF: Reinforced PTFE (PTFE including additives), PE: UHMWPE (Ultrahigh molecular weight polyethylene)

    P/N Appearance Qty Material Remarks Applicable model Links Applicable models
    228-52711-93 1 PE with Backup Ring 40DXR
    40DXS
    40DX3
    40BXR
    40BX3
    30AD
    2040 Plus
    Mikros
    228-51266 1 GFP 20AP
    228-35146 1 GFP 2030
    2010
    2010HT
    20AD
    20AB
    20ADVP
    20ADnano
    opitional for 2040
    3 GFP 2030
    2010
    2010HT
    20AD
    20AB
    20ADVP
    20ADnano
    opitional for 2040
    228-35145 1 GFP 20AT
    10ATVP
    3 GFP 20AT
    10ATVP
    228-32628-91 1 PE 2040
    30ADSF
    20ADnano
    20ADXR
    20ADSP
    optional for 2030
    optional for 2010
    optional for 2010HT
    228-21975-91 1 PE 20AR
    20Ai
    10Ai
    6AD
    optional for 10ATVP
    3 PE 20AR
    20Ai
    10Ai
    6AD
    optional for 10ATVP
    228-18092 1 GF 8A
    3 GF 8A

    Notes for Changing a Material of a Plunger Seal

    When changing a material of a plunger seal, parts other than the seal are also necessary.
    See the details below.

    Seal Material Other Than LC-10AT/LC-10ATVP LC-10AT/LC-10ATVP
    GFP→PE
    GF→PE
    PE seal kit (P/N 228-33233-91)
    A set including the following parts
    • PE seal (P/N 228-32628-91)
    • PVDF seat (P/N 228-33760-92)
    • Cushion for pump head (P/N 228-33230)
    Two types of seats (P/N 228-32325-01 and 228-32401)
    For the LC-10ATVP, the rinsing seal also needs to be changed.
    →Rinsing seal for the LC-10ATVP
    PE→GFP GFP seal kit (P/N 228-35349-91)
    A set including the following parts
    • GFP seal (P/N 228-35146)
    • PVDF seat (P/N 228-33760-92)
    • Cushion for pump head (P/N 228-33230)
    Seal cap (P/N 228-35804)*2)

    *2) For early LC-10ATVP models, P/N 228-35147 (metallic) was used. Currently 228-35804 (trifluoroethylene) is used.

    Rinsing Seal of the LC-10ATVP

    When changing a material of a plunger seal from GFP to PE or changing the rinsing seal for the LC-10ATVP manufactured in June 1998 or earlier to the current seal, the rinsing seal and mounting parts for it also need to be changed.

    Plunger Seal

    When Changing a Material of a Plunger Seal from GFP to PE

    When changing a plunger seal with one made of PE, the rinsing seal and mounting parts for it also need to be changed. The following additional parts are necessary.

    • Rinsing seal P/N 228-28499
    • Seal holder P/N 228-35816-91
    • Ring holder P/N 228-39109-01
    Plunger Seal

    When Changing the Rinsing Seal Used on the LC-10ATVP Manufactured in June 1998 or Earlier to the Current One

    When changing the rinsing seal to the current one (P/N 228-35145), the holder also needs to be changed. Use the P/N 228-35946-91 seal holder assembly (with a seal).

    Suction Filter
    P/N Appearance Qty Remarks Applicable model Links Applicable models
    228-56200-48 1 Assembly (stainless steel) 40BX3
    1 Assembly (stainless steel) Mikros
    1 Assembly (stainless steel) 2040
    2040 Plus
    2030
    2030 Plus
    1 Assembly (stainless steel) 20ADnano
    228-45708-91 1 Assembly (stainless steel) 30ADSF
    30AD
    2030
    2040
    2010
    2010HT
    20ADXR
    20ADnano
    20AR
    20AD
    20AB
    20AT
    6AD
    optional for 10ADVP
    optional for 10ATVP
    228-39181-94 1 Assembly (ceramic) 20ADSP
    20Ai
    10Ai
    228-25079-91 1 Ceramic filter main unit 20Ai
    10Ai
    optional for 20AD
    optional for 10AB
    optional for 20AT
    optional for 10ADVP
    optional for 10ATVP
    228-20031 1 Filter main unit, ESKA-P5375C (stainless steel) 20AP
    8A
    228-18740-95 1 PE optional for 20Ai
    228-17421-91 1 Assembly (stainless steel) 8A
    228-17421-42 1 20AP

    On the assembly, the filter main unit (stainless steel or ceramic), tube, coil spring, ferrule, bush, and other parts are mounted.

    Line Filter
    P/N Appearance Qty Remarks Applicable model Links Applicable models
    228-57501-92 1 40DXS
    40DX3
    40BX3
    228-70394-42 1 Mikros
    228-58173-42 1 with Certificate of Materials 30ADSF
    228-35871-99 1 30AD
    228-35871-96 1 Housing, integrated with a stainless steel filter 2030
    2040
    2010
    2010HT
    20ADXR
    20ADnano
    20AR
    20AD
    20AB
    20AT
    optional for 10ADVP *2)
    optional for 10ATVP *2)
    228-39082-91 1 Line filter, housing 10ADVP
    10ATVP
    228-48607-91 1 PEEK frit 20ADSP
    20Ai
    10ADVP *2)
    10ATVP *2)
    10Ai *2)
    optional for 20AD *1)
    optional for 20AB *1)
    optional for 20AT *1)
    5 PEEK frit 20ADSP
    20Ai
    10ADVP *2)
    10ATVP *2)
    10Ai *2)
    optional for 20AD *1)
    optional for 20AB *1)
    optional for 20AT *1)
    228-33265 1 Line filter, housing 20Ai
    10Ai
    228-12642-93 1 Line filter assembly
    Packing (P/N: 228-12564) is neccessary when replaced.
    6AD
    5 Line filter assembly
    Packing (P/N: 228-12564) is neccessary when replaced.
    6AD
    228-18405-91 1 Line filter (with packing) 20AP
    8A

    *1) Former standard accessories for the LC-20AD/AB/AT
    *2) On the LC-10ADvp/10ATvp, 228-35871-96 is easier to handle than 228-48607-91.

    Check Valve

    ■ Material of the Check Valve
    Unless the material is described in Remarks, the cartridge is made of stainless steel, the ball is made of ruby, and the seat is made of sapphire.

    Name P/N Appearance Qty Remarks Applicable model Links Applicable models
    Check Valve IN 1 40DXS
    40DX3
    40BX3
    Check Valve IN 228-39093-84 3 10ADVP
    Check Valve IN 228-20256-91 1 →Exploded view 8A
    Check Valve IN 1 The cartridge is made of PEEK. 20Ai
    10Ai
    Check Valve IN 3 The ball and seat are ceramic
    →Exploded view.
    optional for 10ATVP *1)
    Check Valve IN 228-35721-91 1 The ball and seat are ceramic
    →Exploded view.
    optional for 10ATVP *1)
    Check Valve IN 3 →Exploded view 10ATVP
    Check Valve IN 228-32166-91 1 →Exploded view 10ATVP
    Check Valve IN 3 The ball and seat are ceramic. optional for 20AD
    optional for 20ADXR
    optional for 20AB
    optional for 20ADSP
    optional for 20ADnano
    optional for 20AT
    Check Valve IN 228-35719-92 1 The ball and seat are ceramic. optional for 20AD
    optional for 20ADXR
    optional for 20AB
    optional for 20ADSP
    optional for 20ADnano
    optional for 20AT
    Check Valve IN 228-39093-92 1 →Exploded view 10ADVP
    Check Valve IN 1 Primary Check valve cartridge Mikros
    Check Valve IN 228-37149-92 1 Check valve cartridge, DIV
    →Exploded view
    2010
    2010HT
    Check Valve IN 228-48249-42 1 In the case of the LC-20AT: Lefy side when viewed from the front Reference: Illustration of LC-20AT Flow Line
    →Exploded view
    20AT
    Check Valve IN 228-20283-91 1 →Exploded view 20AR
    6AD
    Check Valve IN 228-50950-41 1 →Exploded view 20AP
    Check Valve IN 228-52964-95 1 →Exploded view 30AD
    Check Valve IN 228-48249-97 2 →Exploded view 40D
    40DXR
    40BXR
    2030
    2040
    20AD
    20ADXR
    20AB
    20ADSP
    20ADnano
    Check Valve IN 228-48249-96 1 →Exploded view 40D
    40DXR
    40BXR
    2030
    2030 Plus
    2040
    2040 Plus
    20AD
    20ADXR
    20AB
    20ADSP
    20ADnano
    Check Valve IN 1 with Certificate of Material 30ADSF
    Check Valve IN 1 Secondary Check valve Mikros
    Check Valve IN 3 →Exploded view 8A
    Check Valve OUT 1 40D
    40DXR
    40BXR
    Check Valve OUT 1 with Certificate of Meterial 30ADSF
    Check Valve OUT 228-53334-96 1 →Exploded view 40D
    40DXR
    40BXR
    30AD
    Check Valve OUT 228-45705-91 1 →Exploded view 2030
    2040
    20AB
    20AD
    20ADXR
    20ADSP
    20ADnano
    optional for 10ADVP *2)
    optional for 10ATVP *2)
    Check Valve OUT 228-50955-41 1 →Exploded view 20AP
    Check Valve OUT 228-37147-93 1 →Exploded view 2010
    2010HT
    Check Valve OUT 228-34976-91 1 →Exploded view 10ADVP
    10ATVP
    Check Valve OUT 228-34976-81 3 →Exploded view 10ADVP
    10ATVP
    Check Valve OUT 228-32798-91 1 The cartridge is made of PEEK. 20Ai
    Check Valve OUT 3 →Exploded view 10Ai
    Check Valve OUT 228-09054-93 1 →Exploded view 20AR
    8A
    Check Valve OUT 3 6AD

    *1) If acetonitrile (100 % and other high concentrations) is left, a heavy dirt may remain inside the check valve, causing a malfunction. This check valve can operate smoothly even under such circumstances. When using this valve, check the flowrate. The flowrate may need to be corrected.

    *2) When using on the 10ADvp/10ATvp, check the flowrate. Correct the flowrate if necessary.

    Rinsing the Check Valve

    Check valve tends to get dirty easily as solvents go through them. When using a check valve for a long period of time, it is recommended to rinse the valve regularly. Rinse the check valve when the operation of the instrument is stopped after analysis is finished, on weekends, or during a long no operation period.

     

    Rinsing Procedures

    • Prepare a reservoir bottle containing 2-propanol (approx. 200 mL).
    • Connect a resistance tube (inner diameter 0.1 mm, 2 m long) to the outlet of the solvent delivery pump.
    • Replace the mobile phase with 2-propanol by for example using the solvent delivery pump's purge operation.
    • Set the flowrate so that the pressure is at least 10 MPa (standard: 1 mL/min).
    • Deliver 2-propanol for at least 30 minutes.

     

    Flowrate Correction
    On the 20A/VP series, users can correct the flowrate using the VP function.
    Measure the flowrate at the flowrate to be corrected and set the value for [FLOW COMP.FACT] on the [Calibration] menu of the VP function. For details, refer to the "Calibration Support Group" section of the instruction manual.

    Diaphragm, rinsing seal, bearing
    Name P/N Appearance Qty Remarks Applicable model Links Applicable models
    228-55272-41 2 40D
    40DXR
    40DXS
    40DX3
    40BXR
    40BX3
    30AD
    2030
    2040
    2040 Plus
    228-32784-91 2 White, PTFE (polytetrafluoroethylene), more solvent-tolerant and mechanically stronger than 228-24311-01 30ADSF
    20AB
    20AD
    20ADXR
    20ADSP
    20ADnano
    2010
    2010HT
    10ADVP
    228-24311-01 1 Black, Viton® (fluoroelastomer), low solvent-tolerant *1) usable for 10ADVP
    228-24311-84 10 Black, Viton® (fluoroelastomer), low solvent-tolerant *1) usable for 10ADVP
    228-51263 1 20AP
    228-35935 1 Polyethylene, white (translucent) *2) 20AT
    20Ai
    10ATVP
    228-28499 1 Polyethylene, white *2) 10Ai
    3 Polyethylene, white *2) 10Ai
    228-18306 1 PTFE (polytetrafluoroethylene), gray 8A
    5 PTFE (polytetrafluoroethylene), gray 8A
    228-20282 1 PTFE (polytetrafluoroethylene), gray 20AR
    6AD
    1 PTFE (polytetrafluoroethylene), gray 20AR
    6AD
    Rising Seal 1 Mikros

    *1) Viton®is a registered trademark of DuPont Dow Elastomers Japan.

    *2) On the LC-10ATvp units manufactured in June 1998 and later, the rinsing seal 228-35935 is used. On units manufactured earlier and ones with a polyethylene plunger seal, the rinsing seal 228-28499 is used. When changing the seal to a different type, the seal holder assembly also needs to be changed. (Details)

    Supplementary keywords: Plunger, pump, HPLC, high-performance, ion chromatograph, preparative, inert, LC-30A, LC-20A, LC-10A, LC-2010, LC-2030, LC-2040, Prominence, Nexera, Seal, plunger, graphite, Bal, ion, LC-6A, LC-9A, LC-8A, GFP, PE, Suction filter, ion, preparative pump, inert pump, ceramic filter, Solvent delivery unit, LC-10ADvp, LC-10ATvp, LC-10Ai, LC-10AS, LC-10AD, LC-10AT, LC-6AD, LC-2010A, LC-2010C, line filter, Backflow, check valve, ball, seat, check, active inlet valve, LC-7A, rinsing, diaphragm, LC-40D, LC-40DXR, LC-40XS, LC-40DX3, LC-40BXR, LC-40BX3, LC-20AD, LC-20AB, LC-20ADXR, LC-20ADSP, LC-20ADnano, LC-20AT, LC-20Ai, LC-20AR, LC-20AP